BIOM-37. ELUCIDATION OF THE TRANSCRIPTOMIC CONSEQUENCES OF KLF4 AND TRAF7 MUTATIONS IN SECRETORY MENINGIOMAS

Abstract Secretory meningioma (SM) is a benign histological subtype frequently located in the central skull-base, complicating surgical removal, highlighting need of gentle targeting treatment alternatives. SM tumors arise from arachnoid layer meninges and are associated with hotspot p.K409Q mutation KLF4 combined different mutations TRAF7. The objective this study was to identify how TRAF7 alter signaling tumors. includes 16 FFPE samples three cysts as normal-tissue reference. All tissues were histologically confirmed, followed by genomic sequencing KLF4, TRAF7, NF2 TERT-promoter (C220T/C228T) using customized NGS panel. Gene expression profiles analyzed NanoString Pan-cancer Progression Pathways panels, which resulted measurement 1,202 unique transcripts over background level. presented KLF4p.K409Q mutation, 12 had (four being p.N520S), while no these genes. Neither nor or mutations. PCA analysis showed RNA for cysts, without influence on overall gene patterns Sub-analysis found increased TRAF7-mutated versus non-mutated at level, stable all samples. In total 98 genes differentially expressed (54 up- 44 downregulated) log2-fold change 1 an adjusted p-value under 0.05 cysts. Further analyses planned explore validate results. conclusion, we find that correlates more strongly histology, than have expressions distinct normal tissue number disease relevant